高效液相色谱法测定棉籽仁中棉酚的含量

目的：建立棉籽仁中棉酚含量的高效液相色谱测定方法。方法：采用ODS-C18 色谱柱(4.6mm × 200mm，5μm)，柱温25℃，流动相为甲醇- 体积分数1% 磷酸溶液(85:15，V/V)，流速1.0mL/min，检测波长235nm。结果：棉酚质量浓度在1.9～34.9μg/mL 范围内线性关系良好；棉酚的加标回收率为97.8%～100.3%，RSD值为0.4%～1.2%。结论：此法操作简便、快速、灵敏、准确，样品处理简便易行，适于测定棉籽仁中棉酚的含量。

HPLC Determination of Gossypol in Cotton Seed Kernel

A HPLC method was developed for the determination of gossypol in cotton seed kernel. The chromatographic separation was carried out on a ODS-C18 column (4.6 mm × 200 mm, 5 μm) with a mobile phase composed of methanol and 1% phosphoric acid aqueous solution (85:15, V/V) at a flow rate of 1.0 mL/min. The detected wavelength was set at 235 nm. Results showed that a good linear relationship between HPLC peak area and gossypol concentration was obtained over the range of 1.9－34.9 μg/mL. Mean spike recoveries of gossypol at three levels were 97.8%－100.3%, with RSDs ranging from 0.4% to 1.2%. The method provides the benefits of simplicity, rapidity, sensitivity and accuracy, and therefore is applicable to the determination of gossypol in cotton seed kernel.