| 两段双底物发酵生产1,3–丙二醇 |
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| 引用本文： | 杨东,李春,杜晨宇,张延平,曹竹安. 两段双底物发酵生产1,3–丙二醇[J]. 过程工程学报, 2003, 3(3): 269-273 |
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| 作者姓名： | 杨东 李春 杜晨宇 张延平 曹竹安 |
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| 作者单位： | 清华大学化学工程系生物化工研究所,北京,100084 |
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| 基金项目： | “十五”国家科技攻关计划项目(编号:2001BA708B01-04) |
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| 摘 要： | 利用Klebsiella pneumoniae生长与催化耦合的特点，将好氧生长与厌氧转化两个过程耦合，开发了两段双底物发酵生产1,3-丙二醇的新工艺，并对其工艺特点进行了初步研究. 结果表明，采用两段双底物发酵工艺，发酵过程菌体浓度明显高于传统的厌氧转化工艺，OD650最大值达到9.37，发酵60 h, 1,3-丙二醇的浓度达到50.16 g/L，生成速率达0.836 g/(L×h)，比传统工艺提高了45%.
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| 关 键 词： | Klebsiella pneumoniae 1,3-丙二醇；甘油；两段双底物发酵 |
| 文章编号： | 1009-606X(2003)03-0269-05 |
| 修稿时间： | 2003-01-15 |
Production of 1,3-Propanediol by Klebsiella pneumoniae in Two Stage Two Substrate Fermentation |
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| YANG Dong,LI Chun,DU Chen-yu,ZHANG Yan-ping,CAO Zhu-an. Production of 1,3-Propanediol by Klebsiella pneumoniae in Two Stage Two Substrate Fermentation[J]. Chinese Journal of Process Engineering, 2003, 3(3): 269-273 |
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| Authors: | YANG Dong LI Chun DU Chen-yu ZHANG Yan-ping CAO Zhu-an |
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| Abstract: | Two stage two substrate fermentation of 1,3-propanediol by Klebsiella pneumoniae was studied. The fermentation results showed that the cell and 1,3-propanediol concentrations increased to OD650 9.37 and 50.16 g/L respectively. The 1,3-propanediol productivity of 0.836 g/(Lh) was achieved, which is increased by 45% than the conventional anaerobic fermentation. |
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| Keywords: | Klebsiella pneumoniae 1 3-propanediol glycerol two stage two substrate fermentation |
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