高效液相色谱法同时测定食品中18种食品添加剂

建立高效液相色谱同时测定食品中18种食品添加剂的高通量分析方法。对于不含油脂或油脂含量低的样品采用含抗坏血酸棕榈酸酯的正己烷饱和乙腈-6mol/L HCl溶液-饱和氯化钠混合溶液一次提取净化，对于油脂样品采用含抗坏血酸棕榈酸酯的正己烷饱和乙腈-乙腈饱和正己烷液液萃取。采用Ecosil C18色谱柱(250mm×4.6mm，5μm)，乙腈-0.6%乙酸溶液作为流动相，梯度洗脱，用紫外检测器检测，检测波长280nm，外标法峰面积定量。18种食品添加剂在1.0～25mg/L范围内线性良好，相关系数r均大于0.99，样品在10、25mg/kg和50mg/kg三个添加水平的平均回收率为88.9%～99.9%之间，相对标准偏差为2.43%～11.7%(n=6)，方法的定量限为10mg/kg。方法简便、准确，适用于食品中18种食品添加剂高通量的检测。

Simultaneous Determination of 18 Food Additives in Foods by High Performance Liquid Chromatography

A high performance liquid chromatography method was developed for simultaneous determination of 18 food additives in foods. Low fat or fat free food samples were extracted and purified with n-hexane saturated acetonitrile containing ascorbyl palmitate-6 mol/L HCl solution-saturated sodium chloride solution (containing medium fat sample usingnhexane to remove fat). Oil samples were extracted with n-hexane saturated acetonitrile (containing ascorbyl palmitate)- acetonitrile saturated n-hexane. Analysis was performed using HPLC system equipped with an Ecosil C18 column (250 mm × 4.6 mm, 5 μm) and a UV detector at 280 nm with a mobile phase of acetonitrile-0.6% acetic acid solution, at a flow rate of 1.0 mL/min with gradient elution. Good linear was observed in the range of 1.0–25 mg/L with correlation coefficient of 0.99. The average recovery rate in foods was in the range of 88.9%–99.9% at three spiked levels of 10, 25 mg/kg and 50 mg/kg (n = 6), and the relative standard deviation was in the range of 2.43%–11.7%. The limit of quantification was 10 mg/kg. This method is simple, accurate and suitable for the determination of 18 food additives in foods.