高效液相色谱法定量分析茶籽粕中的茶皂素

以脱脂冷榨茶籽饼粕为原料，采用反相ODS填料纯化制备茶皂素标准品，并采用紫外吸收光谱、红外光谱及质谱对该标准品进行鉴定；采用自制标准品为标样，建立一种高效液相色谱法定量分析茶籽饼粕中茶皂素含量的方法。高效液相色谱法条件为反相ODS色谱柱、检测波长215nm、柱温室温、流速1.0mL/min、流动相以乙腈-水体系进行梯度洗脱(0～5min内乙腈体积分数保持为20%，5～10min内流动相乙腈体积分数从20%增至100%，10～15min内流动相乙腈体积分数从100%回至20%)。采用该条件检测得到的茶皂素峰型对称，保留时间合适，杂峰和茶皂素峰分离度较高。

Quantitative Determination of Tea Saponin in Tea Seed Meal by High Performance Liquid Chromatography

In this work, tea saponin was purified by reversed-phase ODS-A column chromatography from degreased coldpressed tea seed meal for use as a reference standard in the quantitative determination of tea saponin by HPLC and identified by UV absorption spectrometry, FTIR and mass spectrometry. HPLC separation was achieved with reversed-phase ODS column and acetonitrile-water as eluent (an initial acetonitrile concentration of 20% held for 5 min, then increased to 100% in 5 min, and finally decreased back to the initial value) at a flow rate of 1.0 mL/min in gradient mode. UV detection was carried out at 215 nm. Under these conditions, symmetric peaks, desired retention time and good separation between tea saponin and impurity were observed.