傅里叶变换红外光谱技术用于细菌检测的影响因素

应用傅里叶变换红外光谱(FT-IR)技术，结合化学计量学分析方法，以大肠杆菌为例，探讨细菌浓度、培养时间、加热灭活等对FT-IR用于细菌检测的影响。结果表明，FT-IR技术可以分别将不同浓度、不同培养时间、活体与加热灭活的细菌进行有效区分，说明细菌浓度、培养时间及加热灭活会影响细菌的光谱特征并干扰FT-IR的细菌检测结果。表明，统一的样品前处理是决定FT-IR检测细菌成功的关键技术之一。

Several Factors for the Detection of Bacteria by FT-IR Spectroscopy

A strain of Escherichia coli was used to study the application of Fourier transform infrared (FT-IR) spectroscopy combined with chemometric method for bacterial detection. We examined the effects of bacterial concentration, culture time and heat inactivation on the detection of Escherichia coli by FT-IR spectroscopy. Results indicated that bacteria with different concentrations, culture time, and heat inactivation could be differentiated by FT-IR spectroscopy, respectively. However, the concentration, culture time and heat inactivation revealed significant impact on spectral characteristics and could interfere with the analytic results. Therefore, uniform preparation of bacteria is one of the most important factors for the successful differentiation of bacteria by FT-IR spectroscopy. Meanwhile, these results provide a theoretical basis for extensive application of FT-IR spectroscopy to detect bacteria.