| 鱿鱼肝脏自水解过程中内源蛋白酶的作用研究 |
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| 引用本文： | 陈 昭,田元勇,马 春,朱蓓薇.鱿鱼肝脏自水解过程中内源蛋白酶的作用研究[J].食品科学,2013,34(7):223-226. |
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| 作者姓名： | 陈 昭 田元勇 马 春 朱蓓薇 |
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| 作者单位： | 大连工业大学食品学院，海洋食品教育部工程研究中心，辽宁 大连 116034 |
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| 基金项目： | 辽宁省重点实验室建设项目(2008403003) |
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| 摘 要： | 对鱿鱼肝脏蛋白酶的酶学性质及其在鱿鱼肝脏自水解过程中的作用进行研究。结果表明：以偶氮酪蛋白为底物，TCA可溶性肽为评价指标，测得鱿鱼肝脏蛋白酶粗酶的最适反应温度为40℃，最适反应pH值为5.0；该粗酶在pH3.0～8.0较为稳定。在鱿鱼肝脏自水解过程中，内源性半胱氨酸蛋白酶发挥主要作用。此外，丝氨酸蛋白酶和金属蛋白酶也有一定作用。
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| 关 键 词： | 鱿鱼肝脏 蛋白酶 自水解 |
| 收稿时间： | 2012-01-19 |
Effect of Endogeneous Proteinases on Autolysis of Squid Hepatopancreas |
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| CHEN Zhao,TIAN Yuan-yong,MA Chun,ZHU Bei-wei.Effect of Endogeneous Proteinases on Autolysis of Squid Hepatopancreas[J].Food Science,2013,34(7):223-226. |
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| Authors: | CHEN Zhao TIAN Yuan-yong MA Chun ZHU Bei-wei |
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| Affiliation: | Engineering Research Center of Seafood, Ministry of Education, School of Food Science and Technology,
Dalian Polytechnic University, Dalian 116034, China |
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| Abstract: | Functional properties of endogeneous proteinases from squid hepatopancreas and their role in the autolysis of
squid hepatopancreas were studied. Proteinases activity was analyzed by determining TCA soluble peptides using azocasein
as a substrate. The results indicated that the optimal pH and temperature were 5.0 and 40 ℃ for crude endogeneous
proteinases, respectively. The enzyme activity remained stable over the pH range of 3.0 to 8.0. The autolysis inhibitory effect
of E-64 proved that the cysteine proteinase played a dominant role in autolysis of squid hepatopancreas. Moreover, other
endogeneous enzymes such as serine proteinase and metallo proteinase also demonstrated varying impacts on the autolysis. |
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| Keywords: | squid hepatopancreas proteinase autolysis |
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