| HPLC法测定桃不同品种果实中花色苷组成和含量 |
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| 引用本文： | 赵秀林,王富荣,徐凌云,何华平,臧 程,田义超.HPLC法测定桃不同品种果实中花色苷组成和含量[J].食品科学,2013,34(8):208-211. |
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| 作者姓名： | 赵秀林 王富荣 徐凌云 何华平 臧 程 田义超 |
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| 作者单位： | 1.武汉工业学院生物与制药工程学院，湖北 武汉 430023；2.湖北省农业科学研究院果树茶叶研究所，湖北 武汉 430209 |
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| 基金项目： | 湖北省农业科技创新中心果树育种专项;武汉工业学院研究生教育创新基金项目 |
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| 摘 要： | 目的： 用高效液相色谱法测定桃不同品种果实中花色苷组成和含量。方法： 色谱柱为C1 8柱(150mm×4.6mm，5μm)，流动相为乙腈：4%磷酸溶液(V/V=12:88，pH2)，检测波长520nm，流速0.8mL/min，进样量20μL，柱温30℃。结果：桃3个品种果实中主要含有1种花色苷：花青素-3-葡萄糖苷，花青素-3-葡萄糖苷在0.4～100μg/mL内线性关系良好(R=0.9992)，低、中、高质量浓度的平均回收率为85.7%、89.4%、91.2%。红、黄、白肉桃果肉中的花青素-3-葡萄糖含量分别为103.7、30.6、21.5mg/kg。结论：本方法操作简单，结果准确可靠，重复性好，可作为桃果实中花青素-3-葡萄糖苷的定量分析方法。
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| 关 键 词： | 桃 花色苷 花青素-3-葡萄糖苷 高效液相色谱法 |
| 收稿时间： | 2012-01-13 |
Anthocyanin Composition and Content in Different Peach Cultivars as Determined by HPLC |
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| ZHAO Xiu-lin,WANG Fu-rong,XU Ling-yun,HE Hua-ping,ZANG Cheng,TIAN Yi-Chao.Anthocyanin Composition and Content in Different Peach Cultivars as Determined by HPLC[J].Food Science,2013,34(8):208-211. |
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| Authors: | ZHAO Xiu-lin WANG Fu-rong XU Ling-yun HE Hua-ping ZANG Cheng TIAN Yi-Chao |
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| Affiliation: | 1. School of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China；2. Institute of Fruit and Tea, Hubei Academy of Agricultural Science, Wuhan 430209, China |
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| Abstract: | A reverse phase HPLC method was utilized to investigate anthocyanin composition and content in different peach cultivars. The calibration curve of cyanidin-3-glucoside was linear (r=0.9992) in the range of 0.4–100 μg/mL, and the recovery rates at lower, median and higher concentration were 85.7%, 89.4%, 92.1%, respectively. Cyanindin-3-glucoside was a major anthocyanin in three different peach cultivars. The cyanidin-3-glucoside contents in blood-flesh, yellow-flesh and white-flesh peach were 103.7, 30.6, 21.5 mg/kg (fresh weight), respectively. This method is suitable for quantitative analysis of cyanidin-3-glucoside in peach. |
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| Keywords: | peach anthocyanin cyandin-3-glucoside HPLC |
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