四君子汤抗辐射活性部位的初步筛选

为筛选四君子汤的抗辐射有效部位,本文通过检测小鼠受照射后3天和7天的外周血象、胸腺系数和骨髓嗜多染红细胞微核率,探讨比较了四君子汤不同提取部位对受照小鼠的辐射损伤防护作用。将6～8周的BALB/c小鼠192只,用随机数字表法分为阴性对照组、照射对照组以及四君子汤水提取物组、乙醇提取物组、水提醇沉50%部位组、水提醇沉60%部位组、水提醇沉70%部位组、水提醇沉80%部位组,以3.5 Gy ⁶⁰Co γ射线全身单次照射造成小鼠电离辐射损伤模型,给药方案为照前连续7天给药,照后连续7天给药。实验结果显示,与照射对照组比较,照后3天,四君子汤水提醇沉80%部位组小鼠的外周血白细胞数显著升高(p<0.05),水提取物、乙醇提取物、水提醇沉50%、60%、70%部位均能显著降低小鼠骨髓嗜多染红细胞微核率(p<0.05);照后7天,四君子汤水提醇沉70%、80%部位组小鼠的胸腺系数显著升高(p<0.05),水提取物、乙醇提取物、水提醇沉60%、70%、80%部位可显著降低小鼠骨髓嗜多染红细胞微核率(p<0.05),其中水提醇沉70%、80%部位组小鼠的微核率最低。上述研究结果表明水提醇沉70%、80%部位可能是四君子汤抗辐射活性的主要部位。此结论为明确四君子汤抗辐射损伤作用的化学成分及抗辐射损伤机制研究奠定了基础,并为开发四君子汤抗辐射中药新药提供了科学依据。

Screening of radiation protective constituents of Sijunzi Decoction

The purpose of this research was to evaluate the radiation protective effects of different constituents of Sijunzi Decoction on BALB/c mice.One hundred and ninety two BALB/c mice aged 6-8 weeks were divided by random number table method into normal control group,model group,Sijunzi Decoction water extract group,alcohol extract group,and groups of the supernatant of 50％,60％,70％,80％ alcohol to precipitate of aqueous extract,respectively.Radiation-damaged mice model were single-irradiated by 3.5 Gy 60Co γ rays,and the extracts of Sijunzi Decoction were administered intragastrically before and after the irradiation.The activity index including peripheral hemogram,thymus index and bone marrow micronucleus rate were examined.The results showed that,compared to the radiation model group,the WBC counts of the group with the supernatant of 80％ alcohol to precipitate of aqueous extract(p ＜0.05) were increased significantly at 3 d after irradiation,while the mice bone marrow micronucleus rates were obviously reduced by the water extract,alcohol extract and the supernatant of 50％,60％,70％ alcohol to precipitate of aqueous extract(p ＜ 0.05).At 7 d after irradiation,the mice thymus indices were elevated in the groups with the supernatant of 70％,80％ alcohol to precipitate of aqueous extract (p ＜ 0.05),whereas the mice bone marrow micronucleus rates were reduced in the same way by the water extract,the alcohol extract as well as the supernatant of 60％,70％,80％ alcohol to precipitate of aqueous extract (p ＜ 0.05).The largest reductions in the mice bone marrow micronucleus rates were observed in the groups of the supernatant of 70％,80％ alcohol to precipitate of aqueous extract.The above results indicate that the supernatant of 70％,80％ alcohol to precipitate of aqueous extract could possibly be the major radiation protective constituents of Sijunzi Decoction.This conclusion provides not only the basis for the studies on the anti-radiation chemical constituents of Sijunzi Decoction and on its anti-radiation effect mechanism,but also the scientific evidence for the new anti-radiation drug development in traditional Chinese medicine.