碳离子束与X射线对女性恶性肿瘤患者外周血淋巴细胞亚群的影响

分析女性乳腺癌及宫颈癌患者外周血在接受不同剂量碳离子束与X射线辐射前后淋巴细胞亚群数值的变化。抽取5例宫颈癌、5例女性乳腺癌患者的外周静脉血,经照射后(设未接受辐射组、1、2 GyE碳离子束组、及1、2 Gy的X射线组)进行淋巴细胞亚群检测。采用独立样本t检验,分别对两组离体血样本进行CD3~+、CD8~+、CD4~+、NK细胞、B细胞、CD4~+/CD8~+两两组间对比,分析数值变化情况。结果显示:两组离体血在接受不同射线、不同剂量照射后,淋巴细胞亚群检测结果差异无显著性的意义(p0.05);接受高线性传能密度(Linear energy transfer,LET)碳离子照射时,乳腺癌组中CD4~+值随剂量增加而升高,宫颈癌组数值则表现出相反的趋势;接受6 MV-X射线照射时,宫颈癌组中NK细胞数值随剂量增加而下降,而乳腺癌组则相反;接受碳离子束照射时,两病种受到1 GyE照射后NK细胞数值均较未照射组数值升高,2 GyE则均较未照射组数值下降。结果提示:女性生殖系统最常见两种恶性肿瘤离体外周血对高LET碳离子和低LET的6 MV-X射线照射表现出了不同的免疫应答反应。

Effects of carbon ion beams and X-rays on peripheral blood lymphocyte subsets in female patients with malignant tumors

Peripheral blood that had been exposed to different doses of carbon ion beams and X-rays in female patients with breast cancer and cervical cancer was analyzed to observe the changes in lymphocyte subsets. The peripheral venous blood from women with cervical cancer (5 patients) and breast cancer (5 patients) was irradiated with carbon ion beams at 1, 2 GyE, X-rays at 1, 2 Gy, and 0 Gy (control), respectively, to test the lymphocyte subgroups. Using independent samplet-test to analyze the changes of CD3+, CD8+, CD4+, NK cells, B cells, and CD4+/CD8+in the two in-vitro blood samples, respectively. The results showed that there were no significant differences (p>0.05) in the values inin-vitro blood of lymphocyte subgroup after exposure to carbon ion beams and X-rays at different doses. The CD4+ value increased with the dose in the breast cancer group and decreased in the cervical cancer group when irradiated with high linear-energy-transfer (LET) carbon ion beams. The NK cell value increased with the dose in the breast cancer group and decreased in the cervical cancer group when irradiated with 6 MV X-rays. The NK cell value was higher in 1 GyE dose group than that in the control group, while lower in 2 GyE dose group when irradiated with carbon ion beams. These results indicate that the in-vitro peripheral blood of the most common two malignant tumors in female reproductive systemirradiated with high LET carbon ions and low LET 6 MV X-rays shows different immune response.