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低能氮离子注入和紫外线复合诱变选育高产酿酒酵母
引用本文:虞龙,姚驰亚,吴晓菲,李玉燕,张娣,虞沐尧. 低能氮离子注入和紫外线复合诱变选育高产酿酒酵母[J]. 辐射研究与辐射工艺学报, 2017, 35(2). DOI: 10.11889/j.1000-3436.2017.rrj.35.020401
作者姓名:虞龙  姚驰亚  吴晓菲  李玉燕  张娣  虞沐尧
作者单位:南京工业大学生物与制药工程学院 南京 210000
基金项目:国家高技术研究发展计划(863计划)(2012AA023400) Supported by the National High-tech Research Development Plan of China
摘    要:通过离子注入联合紫外线辐射诱变选育高产酿酒酵母菌株,根据存活率及突变率确定离子注入的诱变条件为电荷18 ke V,剂量400×10~(14) cm~(-2),紫外线辐射60 s。在该条件下,诱变获得的菌株正突变率和致死率都最高,诱变效果最好。在该条件下进行研究后获得目标菌株SC-N9015,酒精产量从14.65%提升到15.53%。多次传代实验结果显示遗传性状稳定。研究表明,通过低能氮离子注入和紫外线联合诱变选育高产酒精酵母效果显著。

关 键 词:酿酒酵母  低能氮离子注入  紫外线  辐射  诱变筛选

Mutation breeding of ethanol-producing yeast Saccharomyces cerevisiae by low-energy nitrogen ion implantation and UV rays
YU Long,YAO Chiya,WU Xiaofei,LI Yuyan,ZHANG Di,YU Muyao. Mutation breeding of ethanol-producing yeast Saccharomyces cerevisiae by low-energy nitrogen ion implantation and UV rays[J]. Journal of Radiation Research and Radiation Processing, 2017, 35(2). DOI: 10.11889/j.1000-3436.2017.rrj.35.020401
Authors:YU Long  YAO Chiya  WU Xiaofei  LI Yuyan  ZHANG Di  YU Muyao
Abstract:The ethanol-producing yeast Saccharomyces cerevisiae was induced by N+ implantation with an output power of 18 keV and a dose of 400×1014 cm?2,depending on the survival and mutation rates. The UV exposure time was 60 s. Under optimized conditions, the strain achieved the highest positive mutation and lethality rates. A mutant strain SC-N9015 was selected; the ethanol yield of this strain was 15.53%, whereas that of the parental strain was 14.65%. Further, evaluation of ethanol yield over successive generations indicated the hereditary stability of the mutations. The study shows that the combination of low energy N+implantation with UV rays is an efficient method for mutation breeding of S. cerevisiae.
Keywords:Saccharomyces cerevisiae  Low energy N+ implantation  UV rays  Irradiation  Mutation breeding
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