野皂荚多糖修饰羟基磷灰石材料的制备及载药性能研究

靶向载药材料是实现靶向治疗癌症的有效途径之一,新型靶向载药材料的制备和性能提高具有重要研究价值。以四水合硝酸钙、磷酸二氢铵为原料,野皂荚多糖为修饰剂,采用乙醇-水混合溶剂热法,制备得到羟基磷灰石(HAP)材料。用红外(IR)、X射线衍射(XRD)和扫描电镜(SEM)对材料进行结构形貌表征,采用四唑盐(MTT)比色法评价材料细胞毒性,并进行体外载抗癌药物盐酸阿霉素(DOX)性能研究。IR和XRD表征均表明野皂荚多糖修饰剂将多糖有机官能团引入到HAP材料中,野皂荚多糖的结晶成核作用使材料有自组装为棒状花球趋势,所得HAP材料结晶度高,HAP材料对hela细胞基本无毒且对DOX的载药量可高达142.37μg/mg;且所得材料对DOX具有pH响应释放性能,在pH值为7.4和5.0的体外环境下,释放差异15%左右,表明野皂荚多糖修饰的HAP材料具有一定靶向载药的潜在应用价值。

Preparation and drug-delivery study of hydroxyapatite material modified by G. heterophylla polysaccharide

Targeted drug-delivery material is one of the effective ways to treat cancer.Therefore,the study of preparation and performance improvement of novel targeted drug-loading material is very important.Hydroxyapatite(HAP)material was prepared by ethanol-water solvothermal method with calcium nitrate and ammonium dihydrogen phosphate as raw materials and G.heterophylla polysaccharide as modifier.The HAP material was characterized by infrared radiation spectrum(IR),X-ray diffraction(XRD)and scanning electron microscope(SEM).The result shows that polysaccharide organic functional groups were added in HAP materials owing to the G.heterophylla polysaccharide modifier by IR and XRD characterization.The HAP material self-assembled into club-shaped flower ball morphology with G.heterophylla polysaccharide as crystallization nucleation.The crystallinity of HAP material obtained at160℃was higher than that at140℃.Cytotoxicity of HAP materials was evaluated by using tetrazolium salt(MTT)colorimetry.The cell viability was above 95%with concentration in the range of 0.1-100μg/mL.The cytotoxicity test shows that the HAP materials were basically non-toxic to hela cells.The drug-delivery property of HAP material using doxorubicin hydrochloride(DOX)as the anticancer drug was studied.The DOX cumulative drug-loading of functioned HAP materials is up to142.37μg/mg.The specific surface area of HAP material was only 22.15 m 2/g because its dimension was almost more than10 um.It was shown that the high cumulative Dox-loading of obtained HAP material was due to the introduction of polysaccharide functional groups.And the HAP materials had a pH-response release property to DOX.The release tests in vitro of external buffer solutions(pH=7.4 and pH=5.0)were studied and the functioned HAP materials had a targeted drug-delivery performance.The targeted release rate was about15%.These results show that HAP modified by G.heterophylla polysaccharides had potential application value in targeted drug delivery.