A MARANTHUS CRUENTUS LECTIN: PURIFICATION, STABILITY, AND SOME BIOCHEMICAL PROPERTIES |
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| Authors: | ANA M CALDERON DE LA BARCA LUZ VAZQUEZ-MORENO |
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| Affiliation: | Division de Biologia Experimental Centro de Investigaciones Biologicas de B.C.S. La Paz, B.C.S., Mexico;Departamento de Nutricion Centro de Investigacion en Alimentacion y Desarrollo Hermosillo, Sonora, Mexico |
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| Abstract: | Amaranth lectin from Amaranthus cruentus seeds was isolated, purified and partially characterized. The purification method used was based on its ability to interact with fetuin. Effects of temperature, pH and buffers and concentration were determined by measurement of lectin hemagglutination activity. Amaranth lectin biological activity was best maintained at temperatures between 4 and 30°C and pH's above or below 5. It was possible to store amaranth lectin at 4°C in acetate buffers, pH 4.8, for prolonged periods and to concentrate it 10-fold by ultrafiltration with only 18% loss of activity. A subunit molecular weight of 32,500 daltons was determined by SDS-PAGE for amaranth lectin. |
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