Direct evidence for the role of haem doming as the primary event in the cooperative transition of haemoglobin |
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Authors: | S Franzen JC Lambry B Bohn C Poyart JL Martin |
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Affiliation: | Laboratoire d'Optique Appliquée INSERM U275, Ecole Polytechnique-ENSTA, Palaiseau, France. |
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Abstract: | The study of cooperative ligand binding among the four subunits of haemoglobin has played a central role in the understanding of allosteric transitions in a large number of enzymes. Haem iron out-of-plane motion has been suggested to be the trigger for the cooperative transition of haemoglobin. To function as a trigger in a dynamic sense, haem-iron doming must be the first conformational change to occur following ligand dissociation. Here we present the first direct demonstration that haem-iron doming occurs on the same time scale as the breaking of the iron-ligand bond, thus establishing haem-iron doming as the primary event which lead to the R-->T transition in haemoglobin. |
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