复合蛋白酶酶解油菜蜂花粉及其表征

蜂花粉营养成分丰富，并多存在于花粉粒内部，花粉壁的存在限制了蜂花粉的开发和利用。结果表明：采用复合蛋白酶处理花粉，获得最佳破壁效果时的条件为：pH8、48℃、时间48h。并在此条件下利用比表面积、粒度分析、Zeta电位、扫描电镜、透射电镜对蜂花粉破壁进行表征评定，从不同角度反映酶法在改善花粉细胞通透性上起到的作用。

Protamex Hydrolysis and Cell Wall of Rape Bee Pollen

Bee pollen is rich in nutrients, most of which are present inside the cells. The development and exploitation of bee pollen is restricted by the existing cell wall. An investigation to cell wall disruption of rape bee pollen by means of protamex hydrolysis was carried out in this study. The optimal cell wall disruption conditions were found to be: pH 8, 48 ℃ and 48 h hydrolysis. Rape bee pollen with protamex treatment under these conditions was observed under SEM (scan electron microscope) and TEM (transmission electron microscope), and the specific surface area, particle size and Zeta potential were characterized. The results obtained collectively indicate the role of protamex hydrolysis in improving the permeability of rape bee pollen cells from different perspectives.