| 黄参多糖对癌细胞HepG2和P388增殖抑制与细胞凋亡的影响 |
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| 引用本文： | 贾磊,聂秀娟,方梅. 黄参多糖对癌细胞HepG2和P388增殖抑制与细胞凋亡的影响[J]. 食品科学, 2011, 32(19): 227-231. DOI: 10.7506/spkx1002-6630-201119051 |
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| 作者姓名： | 贾磊 聂秀娟 方梅 |
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| 作者单位： | 1.黄山学院体育系2. 甘肃农业大学动物医学院 |
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| 基金项目： | 安徽省教育厅自然科学项目(KJ2009B274Z); 黄山学院引进人才启动项目(2008xskq013) |
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| 摘 要： | 目的：探索黄参提取物黄参多糖(SGP)对肝癌细胞HepG2和淋巴白血病细胞P388增殖的抑制与诱导细胞凋亡的效应，并初步探讨其对生命延长的作用。方法：应用SGP与肝癌细胞HepG2和淋巴白血病细胞P388共培养，采用MTT比色法测定不同剂量SGP对HepG2和P388细胞增殖的抑制；通过对小鼠皮下移植性肿瘤HepG2生长抑制实验，用流式细胞术分析SGP诱导细胞凋亡和细胞周期分布的变化；探讨SGP对荷淋巴白血病小鼠生命延长的作用。结果：SGP可明显抑制体外培养HepG2和P388细胞的增殖，200mg/(kg ·d)剂量作用48h，对HepG2的抑制率可达37.05%(P＜0.01)，而150、200mg/(kg ·d)剂量作用P388细胞48h后抑制率均超过38%(P＜0.01)；SGP在小鼠体内也能明显抑制HepG2移植性肿瘤生长，其抑制率超过40%；可诱导移植性肝癌HepG2细胞凋亡，并使细胞时相分布在G1期发生阻滞；对荷P388(腹水)小鼠生命有延长作用，延长率达10%。结论：SGP对小鼠有良好的抗癌变效果以及对小鼠原发性肝癌和淋巴白血病的良好抑制作用。
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| 关 键 词： | 黄参多糖 HepG2细胞 P388细胞 增殖抑制 细胞凋亡 |
Effect of Sphallerocarpus gracilis Polysaccharides on Proliferation Inhibition and Apoptosis of Sarcoma HepG2 and Mouse Lymphocyte Leukemia P_(388) |
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| JIA Lei,NIE Xiu-juan,FANG Mei. Effect of Sphallerocarpus gracilis Polysaccharides on Proliferation Inhibition and Apoptosis of Sarcoma HepG2 and Mouse Lymphocyte Leukemia P_(388)[J]. Food Science, 2011, 32(19): 227-231. DOI: 10.7506/spkx1002-6630-201119051 |
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| Authors: | JIA Lei NIE Xiu-juan FANG Mei |
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| Affiliation: | (1. Department of Physical Education, Huangshan University, Huangshan 245041, China；2. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China) |
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| Abstract: | Objective: To explore the effect of Sphallerocarpus gracile polysaccharides(SGP) on apoptosis induction and proliferation inhibition of HepG2 and P388 cells as well as the lifespan extension of mice treated with SGP.Methods: HepG2 and P388 cells were co-cultured in the presence of SGP in this study.The inhibitory effect of SGP at different concentrations on the proliferation of HepG2 and P388 was evaluated by MTT assays.The SGP-induced apoptosis and cell cycle change of HepG2 and P388 were analyzed by flow ... |
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| Keywords: | Sphallerocarpus gracilis polysacchorides(SGP) HepG2 cell P388 proliferation inhibition apoptosis |
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