| 嗜酸乳杆菌发酵制备苜蓿叶蛋白的研究 |
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| 引用本文： | 曲敏,杨大鹏,梁金钟,张月学.嗜酸乳杆菌发酵制备苜蓿叶蛋白的研究[J].食品科学,2011,32(19):152-156. |
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| 作者姓名： | 曲敏 杨大鹏 梁金钟 张月学 |
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| 作者单位： | 1.哈尔滨商业大学 食品科学与工程省级重点实验室
2.黑龙江省农业科学院草业研究所 |
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| 基金项目： | 黑龙江省教育厅科学技术研究项目(12511128) |
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| 摘 要： | 以“肇东”紫花苜蓿干草为实验材料，利用嗜酸乳杆菌发酵沉降提取苜蓿叶蛋白，研究嗜酸乳杆菌的生长规律、并筛选高产酸嗜酸乳杆菌，确定苜蓿叶蛋白的等电点；研究发酵时间、接种量、料液比3个因素对叶蛋白得率的影响，确定发酵法提取苜蓿叶蛋白的最佳工艺。结果表明：pH3.0～3.7为苜蓿叶蛋白的最佳沉降范围；最佳发酵条件参数为：发酵时间11h、料液比1:20、接种量为107个/mL；在该条件下，制备的苜蓿叶蛋白粗蛋白含量为45.08%。
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| 关 键 词： | 苜蓿 叶蛋白 嗜酸乳杆菌 发酵 |
Fermenting Water Extract from Young Alfalfa Stems and Leaves with Lactobacillus acidophilus for Protein Precipitation |
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| QU Min,YANG Da-peng,LIANG Jin-zhong,ZHANG Yue-xue.Fermenting Water Extract from Young Alfalfa Stems and Leaves with Lactobacillus acidophilus for Protein Precipitation[J].Food Science,2011,32(19):152-156. |
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| Authors: | QU Min YANG Da-peng LIANG Jin-zhong ZHANG Yue-xue |
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| Affiliation: | (1.Key Laboratory of Food Science and Engineering, Harbin University of Commerce, Harbin 150076, China；
2.Horticultural Sub-academy of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China) |
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| Abstract: | In the present work,we investigated the growth regularity of Lactobacillus acidophilus,screened out a high yield acid-producing strain and used the strain to ferment water extract from the young stems and leaves of Medicago sativa(alfalfa) cv.Zhaodong to precipitate alfalfa leaf protein by produced acids.The precipitation of alfalfa leaf protein from water extract from the young stems and leaves of Medicago sativa(alfalfa) cv.Zhaodong was studied by adjusting its pH.The effects of fermentation time,inoculum... |
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| Keywords: | alfalfa leaf protein Lactobacillus acidophilus fermentation |
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