壳聚糖澄清甜叶菊水提液及其澄清机理探讨

研究壳聚糖絮凝剂在甜叶菊提取液澄清过程中的作用。分别以甜叶菊水提液的澄清率和甜菊苷的损失率为指标，考察不同因素对絮凝效果的影响。通过正交试验法确定壳聚糖絮凝的较优工艺条件为：1%壳聚糖溶液投加量2mL/g甜叶菊干叶、甜叶菊提取液质量浓度1g生药/10mL提取液、pH6.0、温度45℃，絮凝时间4h。在该条件下，甜叶菊水提液的澄清率为86.63%，甜菊苷的损失率为6.89%，与膜分离法澄清的效果相近，且无需特殊设备。与传统化学絮凝法相比，壳聚糖絮凝法能更有效地保留水提液中的有效成分，具有絮凝效果好、易生物降解、对环境安全、无二次污染等优点。

Application of Chitosan Flocculation Method in Clarification of Water Extract from Stevia rebaudiana Bertoni Leaves and Flocculation Mechanism Analysis

The effect of chitosan flocculation on clarifying water extract from Stevia rebaudiana Bertoni leaves,prepared by twice repeated water extraction at a solid/liquid ratio of 1:10(g/mL) followed by pooling and then rotary evaporation concentration of two extracts,was studied.Clarification rate and stevioside loss rate were investigated with respect to different factors.Using orthogonal array design method,the optimal process conditions for chitosan flocculation were determined as follows: ratio between 1% ace...