| 对羟基苯甲酸丙酯对果蝇超氧化物歧化酶(SOD)基因表达的影响 |
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| 引用本文： | 蒋芳平,应琼琼,顾蔚. 对羟基苯甲酸丙酯对果蝇超氧化物歧化酶(SOD)基因表达的影响[J]. 食品科学, 2011, 32(19): 170. DOI: 10.7506/spkx1002-6630-201119037 |
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| 作者姓名： | 蒋芳平 应琼琼 顾蔚 |
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| 作者单位： | 陕西师范大学生命科学学院; |
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| 基金项目： | 中央高校基本科研业务费专项资金项目(GK201002042) |
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| 摘 要： | 探讨对羟基苯甲酸丙酯(PP)对果蝇超氧化物歧化酶(SOD)基因表达的影响,用含不同质量浓度(0.00、0.20、0.50、1.00、2.00g/L)的PP培养基饲喂果蝇10d后,提取不同PP组果蝇总RNA,采用逆转录PCR方法在mRNA表达水平上研究SOD基因。结果表明:PP抑制了果蝇体内抗氧化酶Cu,Zn-SOD、Mn-SOD基因的表达,与对照组相比具有显著性差异(P<0.05);随着PP质量浓度的升高,雌果蝇体内Cu,Zn-SOD基因与雄果蝇体内Mn-SOD基因的表达量随之降低,而Mn-SOD基因在雌果蝇体内总体表达量提高。故PP的毒性作用可能与抑制抗氧化酶基因的表达有关,而这种作用因果蝇性别而异。
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| 关 键 词： | 对羟基苯甲酸丙酯(PP) 果蝇 超氧化物歧化酶(SOD) 基因表达 |
Effect of Propyl p-Hydroxybenzoate on SOD Gene Expression of in Drosophila melanogaster |
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| JIANG Fang-ping,YING Qiong-qiong,GU Wei. Effect of Propyl p-Hydroxybenzoate on SOD Gene Expression of in Drosophila melanogaster[J]. Food Science, 2011, 32(19): 170. DOI: 10.7506/spkx1002-6630-201119037 |
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| Authors: | JIANG Fang-ping YING Qiong-qiong GU Wei |
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| Affiliation: | JIANG Fang-ping,YING Qiong-qiong,GU Wei(College of Life Science,Shaanxi Normal University,Xi,an 710062,China) |
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| Abstract: | In order to explore the effect of propyl p-hydroxybenzoate(PP) on superoxide dismutase(SOD) gene expression in Drosophila melanogaster,PP was used to feed Drosophila melanogaster at the concentrations of 0,0.20,0.50,1.00 g/L and 2.00 g/L for 10 consecutive days,respectively.Total RNA was extracted from Drosophila melanogaster and RT-PCR was used to investigate the impact of PP on mRNA expression level of SOD gene in Drosophila melanogaster.The results suggested that PP could significantly suppress Cu,Zn-SOD... |
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| Keywords: | propyl p-hydroxybenzoate(PP) Grosophila melanogaster superoxide dismutase(SOD) gene expression |
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