GC-MS法分析蔬菜、水果、食用菌中嘧霉胺等4种农药残留及基质效应探讨

建立了GC-MS法同时测定蔬菜、水果、食用菌中嘧霉胺、氟虫腈、哒螨灵、苯醚甲环唑4种农药残留的方法。农药经过乙腈提取,NH2/Carbon复合固相萃取小柱净化,HP-5MS（30m×0.25mm×0.25μm）弹性石英毛细管柱分离后,用GC-MS选择离子模式进行测定及基质匹配标准溶液外标法校准。农药在0.10 mg/kg的添加水平时,4种农药的回收率为87.2%～108.7%,RSD（n=5）为2.2%～6.9%,检出限为2.6～14.9μg/kg。方法适用于蔬菜、水果、食用菌中多种农药残留的测定要求。

Determination of 4 Pesticides Residues Included Pyrimethanil in Vegetables,Fruits and Edible Mushrooms by GC/MS and Discussion of Matrix Effects

A method based on GC/MS was developed to detect 4 pesticides residues included pyrimethanil,fipronil,pyridaben and difenoconazole in vegetables,fruits and edible mushrooms.Pesticides were extracted with acetonitrile,were cleaned up by an NH2/CARBON SPE column,were separated on a capillary column（HP-5MS,30 m×0.25 mm×0.25 μm）,then were determined with GC/MS/SIM and were quantified by extend method of matrix-matched calibration standard.The recoveries of 4 pesticides were in the range of 87.2%~108.7% with RSD（n=5） of 2.2%~6.9% at the spiked level of 0.10 mg/kg,and the limit of detection were 2.6 μg/kg~14.9 μg/kg.This method is suitable for the detection of multi-pesticide residues in vegetable,fruit and edible mushroom.