基于静力称重法的微小容量光学检测系统标定

实验室常用静力称重法对微量液体容量进行检测,该方法在1μL容量的精确度达到2.18%,重复性为1.85%,但该方法无法在实验室外进行。现有的光学测量微量液体容量法的在1μL的精度为6.09%,重复性为1.31%。该法可实现在线检测,但其精度不如静力称重法。对光学测量微量液体容量的方法进行研究分析,提出了一种新的标定容量参照标准的方法,该标定方法将静力称重法与光学测量法两者结合,以静力称重法容量测量结果为参照标准,代替光学法原先的标准液。新的标定方法在原理上将参照标准由点扩展到线,可将光学测量法在10μL点的精度提高到2.014%,重复性1.30%。

Calibration Method for Optical Measurement of Ultra-Micro Liquid Volume

Static weighing method is commonly used in laboratory to measure the volume of micro-liquid,The accuracy of this method is 2.18% and the repeatability is 1.85% for the volume of 1μL. however, it can not be carried out outside the laboratory. The accuracy and repeatability of the existing optical method for measuring micro-liquid volumetry in 1μL are 6.09% and 1.31%. The mentioned method can realize on-line detection, but its accuracy is not as good as static weighing method. A new method for calibrating the capacity reference standard of this method was found by studying and analyzing the method of optical measurement of micro-liquid capacity. The calibration method combines the static weighing method with the optical measurement method, and takes the static weighing method as a new capacity reference standard instead of the original standard liquid of optical method. The new calibration method extends the reference standard from point to line in principle, and can improve the accuracy of optical measurement at 10μL point to 2.014% and repeatability to 1.30%.