| 伏马菌素B1人工抗原的制备及其鉴定 |
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| 引用本文： | 李复辉,樊海新,李寒松,马良友,孙垚,吴金节,王希春.伏马菌素B1人工抗原的制备及其鉴定[J].中国粮油学报,2013,28(11):112. |
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| 作者姓名： | 李复辉 樊海新 李寒松 马良友 孙垚 吴金节 王希春 |
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| 作者单位： | 安徽农业大学,安徽出入境检验检疫局,安徽农业大学,安徽农业大学,安徽农业大学,安徽农业大学 |
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| 基金项目： | 安徽省科技攻关项目（12010402c206）和安徽农业大学大学生创新基金项目（2012191） |
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| 摘 要： | 本试验采用戊二醛一步法将小分子半抗原(伏马菌素B1,FB1)与载体蛋白OVA进行偶联,制备人工抗原FB1-OVA。分别采用聚丙烯酰胺凝胶电泳法(SDS-PAGE)、紫外扫描法(UV)、质谱法(MS)和免疫芯片法来检测FB1与OVA的偶联效果。结果证明,成功得到了人工抗原FB1-OVA,而且通过质谱法检测得出FB1-OVA和OVA的分子量分别是51928.612和44265.718,分析其偶联比为10.6:1。
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| 关 键 词： | 伏马菌素B1 人工抗原 偶联 鉴定 |
| 收稿时间： | 2013/1/22 0:00:00 |
| 修稿时间： | 2013/6/28 0:00:00 |
Preparation and identification of the fumonisin B1artificial antigen |
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| Abstract: | The hapten fumonisin B1 (FB1) was coupled with carrier proteins ovalbumin (OVA) by one-step glutaraldehyde method that to produce the artificial antigen (FB1-OVA). Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), ultraviolet spectroscopy (UV), mass spectrum (MS) and immunoassays method were adopted respectively to determine the conjugate effect of FB1-OVA. The results indicated that the artificial antigen was prepared successfully, the accurate molecular weights of FB1-OVA and OVA were 51928.612 and 44265.718 by MS, and the coupling ratio was 10.6:1. |
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| Keywords: | fumonisinB1 artifical antigen coupling identification |
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