QuEChERS-超高效液相色谱-串联质谱法测定2种茄果类有机蔬菜中三唑酮及其代谢物

目的 建立QuEChERS结合超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)测定2种茄果类蔬菜中三唑酮及其代谢产物三唑醇对映异构体的分析方法.方法 采用乙腈作为提取溶剂,N-丙基乙二胺(...

Determination of triadimefon and its metabolites in 2 kinds of solanaceous organic vegetables by QuEChERS-ulra performance liquid chromatography- tandem mass spectrometry

Objective To establish an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the determination of triadimefon and its metabolite triadimenol enantiomers in two solanaceous vegetables. Methods Using acetonitrile as the extraction solvent, N-propyl ethylene diamine (primary secondary amine, PSA) mixed with graphitized carbon black for purification, separated by CHIRAL ?OD-RH chiral column, UPLC-MS/MS determination, matrix external standard method Quantitative. Results The linear range for triadimefon and triadimenol are 0.02-1.0 mg/L, the correlation coefficients are all above 0.99, and the detection limit (Limits of detection, LOD) is 0.02 mg/L. The average recovery rate of the three addition levels in organic eggplant and organic green pepper was 71.0-97.8%%, and the relative standard deviation (RSD) (n=6) was between 1.2-9.9%. Conclusion The method is simple to operate, has high accuracy and low cost, and can be used to determine triadimefon and metabolite residues in solanaceous organic vegetables.