QuEChERS-气相色谱-三重四极杆-串联质谱法测定果蔬中222种农药残留

目的 建立一种QuEChERS前处理结合气相色谱-三重四极杆-串联质谱法(gas chromatography-triple quadrupole tandem mass spectrometry,GC-MS/MS)测定水果、蔬菜中222种农药残留的分析方法.方法 样品用乙腈均质提取,QuEChERS技术提取和净化,经...

Determination of 222 kinds of pesticide residues in vegetables and fruits by QuEChERS-gas chromatography-triple quadrupole-tandem mass spectrometry

Objective To establish a method for the rapid determination of 222 kinds of pesticide residues in vegetables and fruits by QuEChERS-gas chromatography-triple quadrupole-tandem mass spectrometry (GC-MS/MS). Methods Samples were extracted with acetonitrile, purified by QuEChERS, then separated by gas chromatography, detected by GC-MS/MS in the dynamic multi-reaction monitoring mode (dMRM). The analytes were quantified by matrix-matched standard solutions with internal standard method. Results The results showed that 222 kinds of pesticides had a good linear relationship in the range of 0.005?0.200 mg/L, and the correlation coefficients were greater than 0.9900. The average recoveries of 216 kinds of pesticides in different matrices were in the range of 90%?120% at the spiked level of 0.01, 0.05, 0.10 mg/kg, with relative standard deviations (RSDs) of 1.2%?8.9%. The limits of detection were 0.005?0.010 mg/kg, and the limits of quantification (LOQ) of the method were 0.01? 0.02 mg/kg. Conclution The method is rapid, accurate, sensitive and reproducible, and suitable for screening multiple pesticide residues in a wide variety of vegetables and fruits.