| 人心肌肌钙蛋白Ⅰ的分离纯化 |
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| 引用本文： | 魏景艳,付平平.人心肌肌钙蛋白Ⅰ的分离纯化[J].粉末涂料与涂装,2000,13(4):218-220. |
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| 作者姓名： | 魏景艳 付平平 |
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| 作者单位： | [1]吉林省临床检验中心,长春 [2]白求恩医科大学第三临床学院,长春 |
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| 基金项目： | 吉林省卫生厅科研经费资助 |
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| 摘 要： | 目的 制备抗人cTnⅠ单克隆抗体 ,以便进一步制备AMI诊断制剂。方法 用兔骨骼肌Tnc(sTnC)制备TnC Sepharose 4B亲和柱 ,直接从心肌组织中分离提纯人cTnⅠ。结果 所提纯的cTnⅠ经SDS PAGE分析已达电泳纯 ,其相对分子质量和氨基酸组成与文献报道相符。结论 为建立AMI早期诊断试剂奠定了基础。
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| 关 键 词： | 心肌肌钙蛋白Ⅰ 亲和层析 急性心肌梗塞(AMI) |
Separation and Purification of Human Cardiac Troponin Ⅰ(cTnⅠ) |
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| Wei Jingyan,et al.Separation and Purification of Human Cardiac Troponin Ⅰ(cTnⅠ)[J].Chinese Journal of Biologicals,2000,13(4):218-220. |
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| Authors: | Wei Jingyan |
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| Affiliation: | Wei Jingyan * et al |
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| Abstract: | Objective To prepare the McAb to cardiac troponin Ⅰ(cTnⅠ) for further preparation of diagnostic kit for acute myocardiac infarction(AMI).Methods TnC-Sepharose 4B affinity chromatography column was prepared with rabbit skeletal muscles troponin C(sInC) and used for the direct purification of cTn Ⅰ from human cardiac muscle tissue.Results SDS-PAGE proved that the cTnⅠ was electrophoretic pure, and the molecular weight and amino acid component of it were consistent with that reported in documents.Conclusion The study lays a foundation of developing the diagnostic kit for early AMI. |
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| Keywords: | cTn Ⅰ Affinity chromatography Acute myocardiac infarction(AMI) |
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