| 从包涵体中高效纯化HBx-蛋白 |
| |
| 引用本文： | 颜华,方志正.从包涵体中高效纯化HBx-蛋白[J].粉末涂料与涂装,2000,13(1):27-29. |
| |
| 作者姓名： | 颜华 方志正 |
| |
| 作者单位： | [1]武汉生物制品研究所,武汉 [2]德国国家肿瘤研究所,武汉 |
| |
| 摘 要： | 目的获得具有生物学活性的重组ＨＢｘ－蛋白。方法经ＴＮＦＭＸ缓冲液清洗表达的包涵体，再将 此包涵体变性，复性后，经亲和层析，分步洗脱、收集。结果获得大量高纯度的ＨＢｘ－蛋白，ＨＢｘ－该蛋白的纯度和回 收率分别达到９６％和２８％。结论此方法具有较好的纯化效果，也可应用于其他重组蛋白的制备。
|
| 关 键 词： | 乙型肝炎病毒 x-蛋白 包涵体 亲和层析 |
High Purification of HBx-protein from Inclusion Body |
| |
| Clarus H.Schroeder,Yan Hua,Fang Zhizheng,Clarus H.Schroeder.High Purification of HBx-protein from Inclusion Body[J].Chinese Journal of Biologicals,2000,13(1):27-29. |
| |
| Authors: | Clarus HSchroeder Yan Hua Fang Zhizheng Clarus HSchroeder |
| |
| Abstract: | Objective To obtain recombinant HBx-protein with biological acitivity. Methods After expressed inclusion bodies were washed with TNFMX buffer, the HBx-protein in them were degenerated and re- naturated, then purified by affinity chromatography. Results Large amounts of highly purified x-protein were obtained .The purity and recovery rate of the protein reached 96% and 28% respectively. Conclusion The method established by the authors showed good purification effect. It can also be used for the preparation of other recombinant proteins. |
| |
| Keywords: | Hepatitis B virus x-protein Inclusion body Affinity chromatography |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
