乙型脑炎减毒活疫苗诱导小鼠的细胞免疫应答

目的探讨乙型脑炎(简称乙脑)减毒活疫苗在小鼠中诱导细胞免疫应答的能力,评价疫苗的免疫原性。方法以乙脑减毒活疫苗免疫BALB/c小鼠,制备小鼠脾淋巴细胞,在体外以重组蛋白NS3-1和NS3-2进行刺激,采用酶联斑点法(ELISPOT)检测分泌细胞因子的效应T细胞的频数。结果乙脑减毒活疫苗免疫小鼠的脾淋巴细胞在体外经重组蛋白NS3-1和NS3-2刺激后,可诱导高频数的分泌IFNγ的CD4+和CD8+T淋巴细胞及低频数的分泌IL-4的淋巴细胞;而乙脑灭活疫苗仅诱导低频数的分泌IFNγ和IL-4的T淋巴细胞。结论乙脑减毒活疫苗可在小鼠中有效地诱导NS3-1和NS3-2抗原特异性T淋巴细胞应答,且应答水平显著高于乙脑灭活疫苗。

Induction of Cellular Immune Response by Live Attenuated Japanese Encephalitis Vaccine in Mice

Objective To investigate the activity in induction of cellular immune response in mice and evaluate the immunogenicity of live attenuated Japanese encephalitis (JE)vaccine. Methods BALB / c mice were immunized with live attenuated JE vaccine, and their splenic lymphocytes were isolated and stimulated in vitro with recombinant proteins NS3-1 and NS3-2. The frequency of cytokine-secreting effector cells was determined by ELISPOT. Results After the splenic lymphocytes of mice immunized with live attenuated JE vaccine were stimulated in vitro with NS3-1 and NS3-2, high frequency of IFNγ-secreting CD4+ and CD8+ T lymphocytes and low frequency of IL-4-secreting lymphocytes were induced. However, inactivated JE vaccine induced only low frequency of IFNγ-secreting and IL-4-secreting T lymphocytes. Conclusion Live attenuated JE vaccine induced specific T lymphocyte response against NS3-1 and NS3-2 antigens in mice effectively, and the response level was significantly higher than that induced by inactivated JE vaccine.