| 生物质对红球菌属菌株lawq生长和脱硫酶活力的影响 |
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| 引用本文： | 刘波,熊健,吕浩,何冰芳,应汉杰. 生物质对红球菌属菌株lawq生长和脱硫酶活力的影响[J]. 化学工程, 2005, 33(6): 35-39,47 |
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| 作者姓名： | 刘波 熊健 吕浩 何冰芳 应汉杰 |
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| 作者单位： | 南京工业大学,制药与生命科学学院,江苏,南京,210009;南京工业大学,制药与生命科学学院,江苏,南京,210009;南京工业大学,制药与生命科学学院,江苏,南京,210009;南京工业大学,制药与生命科学学院,江苏,南京,210009;南京工业大学,制药与生命科学学院,江苏,南京,210009 |
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| 基金项目： | 国家十五攻关项目（2001BA707801） |
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| 摘 要： | 红球菌属菌株lawq是专一性脱硫菌,其脱硫酶系由dszA、dszB和dszC组成,其中关键酶是dszC。文章以脱硫活力作为产脱硫酶系的基准,研究了基本营养物质C、N和S对发酵产酶的影响,实验结果发现最佳的碳源、氮源和硫源分别为甘油、氯化铵和二苯并噻吩(DBT),甘油和氯化铵适宜质量浓度为2 g/L,DBT适宜浓度为0.2 mmol/L;还研究了生物质对菌体生长和脱硫酶活力的影响,实验结果发现,添加VB类物质对菌体的生长和酶活力有不同程度的提高。
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| 关 键 词： | 二苯并噻吩 脱硫 红球菌 生物质 |
| 文章编号： | 1005-9954（2005）06-0035-05 |
Effects of biomaterials on the growth of Rhodococcus sp. strain lawq and the activities of desulfurization enzymes |
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| LIU Bo,XIONG Jian,Lü Hao,HE Bing-fang,YING Han-jie. Effects of biomaterials on the growth of Rhodococcus sp. strain lawq and the activities of desulfurization enzymes[J]. Chemical Engineering, 2005, 33(6): 35-39,47 |
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| Authors: | LIU Bo XIONG Jian Lü Hao HE Bing-fang YING Han-jie |
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| Affiliation: | College of Pharmacy and Life Science, Nanjing University of Technology, Nanjing 210009, Jiangsu Province, China |
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| Abstract: | Rhodococcus sp. lawq is the high yield strain of desulfurization enzymes which are composed of dszA, dszB and dszC. Of them dszC is the most important enzyme. Effects of biomaterial on the growth of Rhodococcus sp. strain lawq and the activities of desulfurization enzyme were studied. The results showed that the optimal carbon source, nitrogen source and sulfur source are 2 g/L glycerol, 2 g/L ammonium chloride and 0.2 mmol/L dibenzothiophene respectively. The growth of strain lawq and the activities of desulfurization enzymes are improved weakly by niacin, VBc, VB2, VB1, VB6 and VB12. |
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| Keywords: | dibenzothiophene desulfurization Rhodococcus biomaterial |
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