大孔树脂纯化茶皂素的研究

以脱脂油茶饼粕80％乙醇浸提液为原料，考察了不同大孔树脂对茶皂素液的纯化效果，确定用AB-8树脂进行纯化。研究得出较佳吸附条件：上样液中茶皂素浓度34．4mg／mL、吸附温度25℃、吸附时间100min；较佳脱附条件：脱附温度30℃、洗脱液乙醇浓度80％、洗脱液与上样液体积比4：1、脱附时间80min，在此条件下茶皂素的吸附率90．70％、总洗脱率85．47％，产品纯度85％。

Study on purifying tea saponin by macroporous resin

With the material of 80% ethanol extract of defatted tea seed cake, the purification effect of tea saponin by different macroporous resins is studied. The results show that AB- 8 macroporous resins is the suitable resin for purifying tea saponin. The optimal conditions for the purification of macroporous resin are 34.4 mg/mL of tea saponin concentration of extraction solution, 25 ℃ of adsorption temperature, adsorbing for 100 minutes, 30 ℃ of desorption temperature, 80% of ethanol concentration, 4 ： 1 of the ratio of ethanol and pattern solution, desorbing for 80 minutes. Under the conditions, the adsorption rate of tea saponin is 90.70%, the desorption rate of tea saponin is 85.47% and its purity reaches 85%.