Gel permeation chromatography using controlled-porosity glass. I. Polyacrylamide–formamide solution |
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Authors: | Nobuhiko Onda Kunio Furusawa Noriko Yamaguchi Shgi Komuro |
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Affiliation: | Nobuhiko Onda,Kunio Furusawa,Noriko Yamaguchi,Shūgi Komuro |
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Abstract: | The process of characterizing polyacrylamide and its partially hydrolyzed materials by gel permeation chromatography was examined. The use of controlled-porosity glass and formamide as the stationary phase and the eluent, respectively, resulted in chromatographic behavior in accord with the hydrodynamic volume concept for polyacrylamide fractions. The addition of a salt (KCl) to the eluent was found to retard the elution of the hydrolyzed polyacrylamide. |
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