静电纺胶原/聚环氧乙烷纳米纤维膜的制备及其性能

为改善胶原/聚环氧乙烷纳米纤维膜在液态环境下的结构稳定性,利用静电纺丝技术制备胶原/聚环氧乙烷纳米纤维膜,并用不同浓度的1-乙基-(3-二甲基氨基丙基)碳二亚胺盐酸盐/N-羟基琥珀酰亚胺对其进行交联改性,对其在液态环境下的溶胀性能、干湿态力学性能、溶血及凝血性能进行测试与分析。结果表明:经交联改性后,胶原/聚环氧乙烷纳米纤维膜在液态环境下浸泡7 d后仍可保持良好的纳米纤维形貌,纤维的溶胀率低于180%,力学性能得到明显改善;交联改性后的纤维膜溶血率均远低于2%,不会对红细胞造成破坏,且凝血性能得到明显改善,凝血指数由未交联的48%降低至20%以下。

Preparation and properties of electrospun collagen/polyethylene oxide nanofiber membranes

In order to maintain and improve the structural stability of the collagen/polyethylene oxide nanofiber membranes under liquous conditions,the improved collagen/polyethylene oxide nanofiber membranes were prepared through electrospinning and by modifying the polymer mix with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide.The degree of swelling ratio,mechanical performance in dry and wet states,hemolytic performance and blood clotting property of the nanofiber membranes were measured and analyzed.The results show that the nanofibers is able to maintain its morphology after being soaked in phosphate buffered saline for 7 d with the swelling of fiber reduced(lower than 180%)and mechanical performance improved.In addition,the hemolysis percentage of crosslinked nanofiber membranes were considerably lower than 2%,indicating no damage to the red blood cells.More importantly,the blood clotting index of all crosslinked nanofiber membranes were all lower than 20%when compared to 48%with a non-crosslinked sample,indicating superior clotting property.