| 野生曲茎石斛的离体培养及再生体系研究 |
| |
| 引用本文： | 余晓丽,王世茹,曾万勇,李佳. 野生曲茎石斛的离体培养及再生体系研究[J]. 武汉工业学院学报, 2010, 29(1): 21-24. DOI: 10.3969/j.issn.1009-4881.2010.01.007 |
| |
| 作者姓名： | 余晓丽 王世茹 曾万勇 李佳 |
| |
| 作者单位： | 1. 武汉工业学院,生物与制药工程学院,湖北,武汉430023
2. 河南南阳农校园艺系,河南,南阳473000 |
| |
| 基金项目： | 武汉工业学院校立科研项目 |
| |
| 摘 要： | 以野生的曲茎石斛茎段为试材，探讨了不同生长调节剂对其不定芽诱导、增殖和生根的影响，并建立了野生曲茎石斛的高效再生体系。结果表明，利于野生曲茎石斛分化的诱导培养基为M（B5）＋6-BA2．0mg／L＋NAA0．8mg／L＋3．0％蔗糖；以MS＋6-BA2．0mg／L＋NAA1．2mg／L＋3．0％蔗糖为增殖培养基时的增殖效果较佳，增殖倍数为5．6；野生曲茎石斛的高效再生体系为MS＋KT1．5mg／L＋NAA1．0mg／L＋IBA1．0mg／L＋2．0％蔗糖，其生根率可达94％；移栽基质以小树皮＋碎火山石＋椰壳粉（1．2：1．2：1）为佳，移栽成活率可达到94％。
|
| 关 键 词： | 曲茎石斛 组织培养 快速繁殖 植物生长调节剂 |
Culturing of wild Dendrobium flexicaule and studing of regeneration system |
| |
| YU Xiao-li,Wang Shi-ru,Zeng Wang-yong,Li Jia. Culturing of wild Dendrobium flexicaule and studing of regeneration system[J]. Journal of Wuhan Polytechnic University, 2010, 29(1): 21-24. DOI: 10.3969/j.issn.1009-4881.2010.01.007 |
| |
| Authors: | YU Xiao-li Wang Shi-ru Zeng Wang-yong Li Jia |
| |
| Affiliation: | YU Xiao-li1,Wang Shi-ru2,Zeng Wang-yong1,Li Jia1 (1.School of Biology , Pharmaceutical Engineering,Wuhan Polytechnic University,Wuhan 430023,China,2.Horticulture Department of Nang Yang Agriculture School,Nanyang 473000,China) |
| |
| Abstract: | By taking wild Dendrobium flexicaule as explants,we studied the influence of different growth regulating substances on adventitious buds induction,multiplication and rooting,and established regeneration system of high efficiency on Dendrobium flexicaule.The results suggested that the best medium for induce was M(B5)+6-BA 2.0 mg/l +NAA 0.8 mg/l +3.0%sucrose;the best medium for the multiplication of medium was the best with MS +6-BA 2.0 mg/l + NAA 1.2 mg/l +3.0%sucrose,the multiplication multiple was 5.6;the ... |
| |
| Keywords: | Dendrobium flexicaule tissue culture rapid propagation plant growth regulator(PGR) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
