蜂胶对氡及其子体吸入致大鼠损伤的防护作用研究

研究蜂胶对氡及其子体吸入致大鼠血细胞与肺氧化应激损伤的防护效应。采用雄性Wistar大鼠28只，随机分为7组，每组4只。染氡＋蜂胶组大鼠于每次吸入氡及其子体前灌服蜂胶0．2g／kg。除对照组外，各处理组整体暴露于多功能生态氡室，吸入氡及其子体的累积剂量分别达到30、67、111工作水平月（Working level month，WLM）后，检测各组大鼠外周血以及肺组织匀浆的超氧化物歧化酶（Superoxide dismutase，SOD）、丙二醛（Malonic dialdehyde，MDA）含量。结果表明，大鼠的外周血以及肺组织中SOD含量，随着氡及其子体染氡剂量的增加而降低，而MDA的含量呈现相反方向的变化。与30WLM染氡组相比，30WLM染氡＋蜂胶组大鼠肺匀浆MDA含量显著降低。与67WLM染氡组相比，67WLM染氡＋蜂胶组大鼠肺匀浆SOD活性显著提高，外周血和肺匀浆MDA含量显著降低。与111WLM染氡组相比，111WLM染氡＋蜂胶组大鼠外周血和肺匀浆SOD活性显著提高，MDA含量显著降低。在本实验条件下，氡及其子体吸入可引起大鼠的氧化损伤。蜂胶能够增强大鼠的抗氧化应激能力。

The protective effect of propolis on damage to lung and blood in rats by inhaled radioactive radon and its progeny

Twenty-eight male wistar rats were randomly divided into seven groups, i.e. the radon groups (3), the propolis radon groups (3) and the control (1). The propolis radon groups were fed intragastrically with propolis 0.2g/kg, before exposing them, together with the radon groups, to radon and its peogeny with the cumulative dose up to 30, 67 and 111 working level month(WLM), respectively. The levels of SOD (superoxide dismutase) and MDA (Malonic dialdehyde) in blood and lung tissue were determined. The SOD level of in blood and lung tissues of the radon groups decreased significantly and the MDA level increased. The MDA level in lung tissue of the 30 WLM propolis radon group was significantly higher than the 30 WLM radon group. The SOD level in lung tissue of the 67 WLM propolis radon group was significantly higher, but the MDA level was significantly lower, than the 67 WLM radon group. Both the SOD and MDA levels in blood and lung tissue of the 111 WLM propolis radon group were significantly higher than the 111 WLM radon group. In conclusion, the inhallation of radon and its progeny can lead to persistent disturbance of the redox state in rats. Propolis show some protective effects on the redox damage under the experimental conditions.