| 保加利亚乳杆菌超浓缩培养过程中的透射电镜观察 |
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| 引用本文： | 李霞,刘尚军,高学军,袁肖寒.保加利亚乳杆菌超浓缩培养过程中的透射电镜观察[J].中国乳品工业,2007,35(3):14-16. |
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| 作者姓名： | 李霞 刘尚军 高学军 袁肖寒 |
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| 作者单位： | 1. 南阳理工学院,生物与化学工程系,河南,南阳,473004
2. 东北农业大学,生命科学与生物技术研究中心,哈尔滨,1500030 |
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| 摘 要： | 采用改良MRS培养基及二次培养对保加利亚乳杆菌(L.b)进行了超浓缩培养,测定了超浓缩培养过程中菌体的生长曲线和乳酸含量变化曲线,用超薄切片透射电镜系统地观察了在培养过程中不同时间菌体的超微结构变化。结果表明,保加利亚乳杆菌超浓缩培养过程中存在明显的菌体形态变化,与菌体能量代谢和产酸规律密切相关。
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| 关 键 词： | 保加利亚乳杆菌 超浓缩培养 电镜观察 生长曲线 乳酸 |
| 文章编号： | 1001-2230（2007）03-0014-03 |
| 修稿时间： | 2006-04-27 |
Transmission electron microscopic observations of the Lactobacillus bulgaricus during super-concentrated incubation |
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| LI Xia,LIU Shang-jun,GAO Xue-jun,YUAN Xiao-han.Transmission electron microscopic observations of the Lactobacillus bulgaricus during super-concentrated incubation[J].China Dairy Industry,2007,35(3):14-16. |
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| Authors: | LI Xia LIU Shang-jun GAO Xue-jun YUAN Xiao-han |
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| Affiliation: | 1.Department of biology and chemistry ,Nanyang Institute of Technology,Nanyang 473004,China; 2. Research Center of Life Science and Biotechnology , Northeast Agricultural University , Harbin 150030 , China |
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| Abstract: | Lactobacillus bulgaricus(L.b) was incubated to high cells population by modified MRS culture micro-filtration membrane , and growth curve and LD content of the bacteria at different time were determined respectively during the incubation.The changes of ultramicrostructure of L.b were systematically observed by ultrathin section TEM at different time during super-concentrated incubation.The results show that microscopic morphology changes of bacteria exist obviously during super-concentrated incubation in culture, and they are related to the rule of energy metabolism and acid producing. |
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| Keywords: | Lactobacillus bulgaricus super-concentrated incubation transmission electron microscopic(TEM) observations growth curve lactic acid |
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