Isolation and characterization of an alkaliphilic bacterium utilizing pyrene as a carbon source |
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Authors: | Habe Hiroshi Kanemitsu Mieko Nomura Mizuki Takemura Tetsuo Iwata Kenichi Nojiri Hideaki Yamane Hisakazu Omori Toshio |
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Affiliation: | Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. uhhabe@mail.ecc.u-tokyo.ac.jp |
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Abstract: | Alkaliphilic Mycobacterium sp. strain MHP-1, which can grow on pyrene as a sole carbon and energy source, was isolated from a soil sample. At the optimum pH for growth (pH 9), about 50% of pyrene (final concentration at 0.1% [w/v]) was degraded during 7 d of incubation, and 4,5-phenanthrenedioic acid, 4-phenanthroic acid and phthalic acid were identified as metabolic intermediates. Strain MHP-1 was found to possess aromatic-ring dioxygenase genes, which are highly homologous to the known nidAB genes from pyrene-degrading mycobacteria. |
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