| 荧光RT-PCR与常规RT-PCR检测禽流感病毒比较研究 |
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| 引用本文： | 宫爱艳 吴胜昔 胡仁健 王健. 荧光RT-PCR与常规RT-PCR检测禽流感病毒比较研究[J]. 重庆理工大学学报(自然科学版), 2006, 20(11): 8-10,49 |
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| 作者姓名： | 宫爱艳 吴胜昔 胡仁健 王健 |
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| 作者单位： | 重庆工学院生物工程学院 重庆400050(宫爱艳，吴胜昔，胡仁健)，重庆市农业局 重庆400015(王健) |
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| 基金项目： | 重庆市防治高致病性禽流感应急专项课题资助项目（渝科发字[2004]9号）. |
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| 摘 要： | 采用目前公认的2种敏感特异的检测方法———荧光RT-PCR和常规RT-PCR———检测标准毒株禽流感病毒H9亚型(鸡胚尿囊液)及部分待检样品,进行灵敏度、特异性、安全性等方面的比较.结果表明:荧光RT-PCR方法在4 h内就能得出结果,检测尿囊液的禽流感病毒灵敏性达到10-7;RT-PCR方法6 h出结果,其灵敏度为10-5,且荧光RT-PCR不需电泳,不接触EB,更安全.因此,荧光RT-PCR比常规RT-PCR更灵敏、快速和安全.
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| 关 键 词： | 禽流感 RT-PCR 诊断 |
| 文章编号： | 1671-0924（2006）11-0008-03 |
| 收稿时间： | 2006-09-14 |
| 修稿时间： | 2006-09-14 |
Comparative Study on Fluorescence RT-PCR and RT-PCR Testing Methods for Detecting Avian Influenza Virus in Chickens |
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| GONG Ai-yan, WU Sheng-xi, HU Ren-jian, WANG Jian. Comparative Study on Fluorescence RT-PCR and RT-PCR Testing Methods for Detecting Avian Influenza Virus in Chickens[J]. Journal of Chongqing University of Technology(Natural Science), 2006, 20(11): 8-10,49 |
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| Authors: | GONG Ai-yan WU Sheng-xi HU Ren-jian WANG Jian |
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| Affiliation: | 1. Bioengineering College, Chongqing Institute of Technology, Chongqing 400050, China; 2. Agricuhural Bureau of Chongqing, Chongqing 400015, China |
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| Abstract: | An experiment with the allantoic fluid of AIV H9 subtype and chicken samples is conducted to compare two accepted sensitive methods of fluorescence RT-PCR and RT-PCR on sensitivity,specificity and reliability.Fluorescence RT-PCR AIV could be detected in four hours with a dilution of 10~(-7) allantoic fluid of AIV H9 subtype;RT-PCR test could detect with only a dilution of 10~(-5) allantoic fluid of AIV H9 subtype in six hours.Fluorescence RT-PCR has an advantage of omitting the step of electrophoresis,which makes it safer than RT-PCR without getting touch with EB.Here a conclusion can be safely drawn that fluorescence RT-PCR appears to be more sensitive,faster and safer than the RT-PCR methods for detection of AIV. |
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| Keywords: | Avian Influenza RT-PCR diagnosis |
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