| 蓝莓叶片原生质体的制备 |
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| 引用本文： | 王丹丹,郭文坛,蒋健,李志新. 蓝莓叶片原生质体的制备[J]. 辽东学院学报(自然科学版), 2011, 0(2): 150-153 |
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| 作者姓名： | 王丹丹 郭文坛 蒋健 李志新 |
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| 作者单位： | 辽东学院农学院,辽宁丹东,118003 |
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| 摘 要： | 文章以北陆蓝莓叶片为材料,研究酶液配比浓度、酶解时间、温度、pH值以及甘露醇浓度对蓝莓叶片原生质体制备的影响.结果显示,以2.0%纤维素酶+0.8%果胶酶为酶液组合,0.60 mol/L甘露醇为渗透压稳定剂,酶液pH值为5.8,温度(26±1)℃,黑暗条件下酶解8h,此后以1000r/min,离心6 min,所得蓝莓叶...
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| 关 键 词： | 北陆蓝莓 叶片 原生质体 |
Preparation of Protoplast from Leaves of Northland Blueberry |
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| WANG Dan-dan,GUO Wen-tan,JIANG Jian,LI Zhi-xin. Preparation of Protoplast from Leaves of Northland Blueberry[J]. Journal of Liaodong University(Natural Sciences), 2011, 0(2): 150-153 |
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| Authors: | WANG Dan-dan GUO Wen-tan JIANG Jian LI Zhi-xin |
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| Affiliation: | (College of Agriculture,Eastern Liaoning University,Dandong 118003,China) |
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| Abstract: | The effect of enzyme solution proportion and density,enxymolysis time,temperature,pH value and manitol density on protoplast preparation from leaves of northland blueberry was studied.The results showed that the optimal enzyme solution for protoplast isolation was 2.0% cellulose added with 0.8% pectolgase with 0.60 mol/L mannitol as the osmosis stabilizer.The pH of enzyme solution was 5.8 and temperature was(26±1) ℃.Enzymolysis time was 8 h in dark.Further more,centrifuging the yield at speed of 1 000 rpm for 6 min. |
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| Keywords: | northland blueberry leaves protoplast |
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