Diagnosis of Liposcelis entomophila (Insecta: Psocodea: Liposcelididae) based on morphological characteristics and DNA barcodes |
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| Authors: | Qianqian Yang Zuzana Ku?erová Zhihong Li Irma Kalinovi? Václav Stejskal George Opit Yang Cao |
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| Affiliation: | 1. Department of Entomology, College of Agronomy and Biotechnology, China Agricultural University, Yuanmingyuan West Road 2, Beijing 100193, PR China;2. Crop Research Institute, Drnovská 507, 161 06 Prague 6, Czech Republic;3. Faculty of Agriculture in Osijek, Department for Plant Protection, University of Josip Juraj Strossmayer in Osijek, Trg Sv. Trojstva 3, 31000 Osijek, Croatia;4. Department of Entomology and Plant Pathology, Oklahoma State University, 127 Noble Research Center, Stillwater, OK, USA;5. Academy of State Administration of Grain, No. 11 Baiwanzhuang Street, Beijing 100037, PR China |
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| Abstract: | Liposcelis entomophila infests stored grain and is one of the most important psocid species worldwide. Six geographically isolated strains of L. entomophila from Asia, Europe, and United States of America (USA) were compared based on morphological attributes and by molecular methods. Decisive characters of morphological diagnosis were studied using body size measurements and Scanning Electron Microscopy (SEM). Molecular identification of the six strains was performed via identification of DNA sequence similarities and phylogenetic analyses based on a 655-bp fragment from the 5′ end of the standard mitochondrial gene cytochrome c oxidase I (COI) barcode region. The results showed that both morphological and molecular approaches were able to accurately identify this species. Kimura-2-Parameter (K2P) divergence between geographically isolated strains was on average 1.75% for the COI sequence. Phylogenetic analyses revealed that sequences of L. entomophila strains' COI barcodes formed clusters with tight cohesion that were clearly distinct from those of allied species. |
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