Kinetic study of a hen-egg white lysozyme-based oenological preparation |
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Authors: | Esti Marco Liburdi Katia Palumbo Fabio Benucci Ilaria Garzillo Anna Maria Vittoria |
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Affiliation: | 1. Department for Innovation in Biological, Agro-food and Forest systems (DIBAF), Tuscia University, via S. Camillo de Lellis, 01100, Viterbo, Italy 2. Department of Ecological and Biological Sciences, Tuscia University, Largo dell’Universit, 01100, Viterbo, Italy
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Abstract: | The catalytic activity of a lysozyme-based oenological preparation toward the specific substrates i) glycol chitosan, as a non-cellular synthetic substrate, and ii) Oenococcus oeni, as a microbial substrate for muramidase activity, was investigated in wine-like acidic medium (tartaric buffer, pH 3.2). The good reproducibility and reliability of results revealed that both substrates are useful for lysozyme kinetics studies. Both the chitinolytic and muramidase activity of lysozyme were affected by uncompetitive substrate inhibition. However, the O. oeni effect occurred at very high concentration with respect to the typical wine bacterial content, even when microbial population reaches its maximum growth level during malolactic fermentation. The optimum pH and temperature for lysozyme activity were very far from the usual wine handling conditions, but residual enzyme activity was observed at pH 3.2 and 20°C, confirming that the oenological application of lysozyme is a useful practice. |
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