香樟木质部挥发性成分的SPME-GC/MS分析

采用固相微萃取技术（SPME）吸附香樟木质部挥发性成分，结合气相色谱-质谱（GC/MS）进行化学成分的分离和鉴定，同时比较不同色谱柱对分离效果的影响。结果表明：采用弱极性DB-5MS色谱柱共分离出51个组分，解析出43种挥发性成分，占总峰面积的97.04%，分离效果和所得的峰形较好于DB-WAXetr色谱柱。鉴定出含量较高的成分有：左旋-α-蒎烯（4.57%）、莰烯（2.14%），双环[3.1.0]-4-甲基-1-异丙基-2-己烯（4.16%）、β-蒎烯（2.3%）、邻异丙基甲苯（2.15%）、D-柠檬烯（7.49%）、桉叶油醇（13.85%）、樟脑（38.71%）、(R)-4-萜品醇（1.74%）、α-松油醇（2.40%）、黄樟素（2.96%）、α-荜澄茄油烯（4.36%）、1-石竹烯（1.92%）等。该方法可为香樟材在医药、食品和化学工业等方面的进一步开发和利用提供参考。

Analysis of Volatile Components in Cinnamomum camphora Xylem by SPME-GC/MS

The volatile components of Cinnamomum camphora xylem were extracted by solid phase microextraction(SPME) and were identified by gas chromatography-mass spectrometry (GC/MS). The separation efficiency of different chromatographic column was compared as well. The results show that GC/MS spectrometric analysis with DB-5MS isolates 51 components, which contains 43 kinds of volatile content, and the proportion of the peak area of volatile components is 97.09%. The separation efficiency and the peak shape of GC/MS spectrometric analysis with DB-5MS are superior to that with DB WAXetr. Based on the identification of the volatile components, (1S)-α-pinene(4.57%), camphene(2.14%), bicyclo[3.1.0]-4-methyl-1-(1-methylethyl)-hex-2-ene(4.16%), β-pinene(2.3%), 1-methyl-2-(1-methylethyl)-benzene(2.15%), D-limonene(7.49%), eucalyptol(13.85%), camphor(38.71%), （R）-4-terpineol(1.74%), α-terpineol(2.40%), safrole(2.96%), α-cubebene(4.36%) and caryophyllene(1.92%) account for most of them. The method can provide a reference for the further development and utilization of Cinnamomum camphora in medicine， food and chemical industry，etc.