A solvent extractor system for the rapid extraction of lipids and trace bioactive micronutrients in oilseeds |
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Authors: | J A Singleton L F Stikeleather |
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Affiliation: | (1) JSDA, ARS, Market Quality and Handling Research, North Carolina State University, 27695-7625 Raleigh, North Carolina;(2) Department of Biological and Agricultural Engineering, North Carolina State University, 27695-7625 Raleigh, North Carolina |
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Abstract: | A low-cost laboratory extractor has been designed and constructed that selectively extracts polar and nonpolar components
from oilseeds and other matrices. The extractor uses available high-performance liquid chromatography laboratory equipment
for pumping the solvent into the extractor. Pressure, temperature, and valving arrangements are automatically controlled by
commercially available components. Advantages of this system include low initial investment, reduced solvent consumption,
shorter extraction times, quantitative lipid recovery, use of multiple extraction solvents, and reduction in cost per sample.
The method has broader applications that include extraction of trace components from a variety of matrices, for example, the
extraction of pesticides from foods and polychlorobiphenyls from soil. Class separation of components from different matrices
can be achieved easily by selection of solvents with the appropriate polarity characteristics. Very small samples can be extracted
simply by changing cell size or by adding an inert material to the cell to fill the void volume. Analyte collection can be
accomplished by collecting in a test tube with an appropriate solvent, or on a solid-phase material. Optimization of extraction
times, number of extractions, matrices, and solvent used is described. Neutral lipids were extracted from peanut meal in 70
min by the rapid extraction method compared to 1440 min required to extract the comparable amount of neutral lipids from a
similar sample by the Soxhlet extraction method. |
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Keywords: | Design equipment evaluation matrix oil quantitation rapid extraction solvent |
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