柠檬醛对阿糖胞苷抗白血病细胞HL-60强效增敏作用研究

通过对柠檬醛与临床首选药物之一阿糖胞苷联用抗白血病细胞株HL-60的研究,探索二者联用的生物效应及其作用机制。采用MTT和DAPI核染色等方法观察柠檬醛同阿糖胞苷的不等浓度组合HL-60的增殖抑制和诱导凋亡作用,相分析电泳激光散射技术检测联用对细胞表面电荷的影响。柠檬醛显示一定的抑制HL-60增殖,促进其凋亡的效应,并与阿糖胞苷有较显著的协同性,增加柠檬醛浓度与药物阿糖胞苷联合作用可使膜表面电位下降的幅度增大。柠檬醛有联合阿糖胞苷临床治疗潜力。

Preliminary study of citral enhance sensitivity and efficiency on Ara-C-treatment of leukemia HL-60

To investigate the inhibitory effects of citral combined with Ara-c,which is one of preferred clinical drugs,on leukemia cell line HL-60,and to study their coordination and possibility of citral as multidrug-resistance（MDR） reversal agent in treatment of leukemia.Apoptosis of HL-60 cells was identified and analyzed by MTT assay,DAPI etc,Phase Analysis Light Scattering was used to measure the surface charges（Zeta potential）,which has been treated by drug conbination.Different concentrations of citral could suppress the proliferation and induced the apoptosis of HL-60 cells in a does dependent manner,and erased more negetive charges on cell membrane with higher coordination of citril combined with Ara-c.Citral had a notable cooperativity with Ara-c.Citral had the potentinlity to acute leukemia in clinic therapy combined with Ara-c.