纳米金基因芯片技术快速检测丝状真菌的研究

目的：建立并评价一种快速检测和鉴定丝状真菌的方法。方法：利用通用引物一次PCR法扩增待检丝状真菌核糖体基因内部间隔区2序列,扩增产物与基因芯片上特异性探针杂交,杂交信号通过纳米金标记和银染反应放大形成裸眼可见的显色信息来判读检测结果。结果：通用PCR扩增方法成功扩增出11种受试菌株的靶DNA,所设计选用的各探针特异性强、可靠性好。所有模拟样品均获得正确的鉴定。结论：该技术方法操作简单、快速、不需要特殊设备,能部分满足临床检测通量的基本要求,具有很好的临床应用前景。

Rapid detection of mold fungus by nanogold-baesd DNA microarray

Objective： To set up and evaluate a method for rapid detection and identification of mold fungus.Methods： The internal transcribed spacer two fragments of fungal ribosomal DNA were amplified using a polymerase chain reaction.Next,the products were hybridized with the probes immobilized on the surface of a microarray.The hybridizing signals were enhanced with gold nanoparticles and silver deposition,and detected using a flatbed scanner or visually.Results：Universal primers can amplify the rRNA gene from mold fungus.All selected probes were of strong specificity and great reliability.All clinical samples spiked with mold fungus were identified correctly.Conclusions： This method is a simple, fast,sensitive technology and do not need special equipment.It can meet the flux demand of clinical detection and has good prospect of clinical application.