| 大豆灰斑病1号生理小种抗性基因的RAPD标记 |
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| 引用本文： | 董伟,杨庆凯.大豆灰斑病1号生理小种抗性基因的RAPD标记[J].高技术通讯,1999,9(10):48-51. |
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| 作者姓名： | 董伟 杨庆凯 |
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| 作者单位： | [1]中国科学院遗传研究所植物生物技术开放实验室 [2]东北农业大学大豆研究所 |
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| 摘 要： | 以高抗品种东农９６７４为父本，以高感品种东农８７－１０４为母本，杂交得到Ｆ２代群体。该群体经人工接种灰斑病１号生理小种后，分别从抗、感材料中取１５株的叶片提取ＤＮＡ组成抗，感池，用８２０个１０ｍｅｒ随机引物进行ＲＡＰＤ多态性分析，其中３个引物在抗，感池间出现稳定的多态性标记ＯＰＫ０３８４０，ＯＰＭ１７１７００，ＯＰＯ１０９５０，并在Ｆ２代个体中表现出抗性与多态性标记协同分离的趋势，这三个标记与抗性
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| 关 键 词： | 大豆 灰斑病 抗性基因 RAPD 分子标记 育种 生理 |
Identification of RAPD Markers Linked to Resistance Gene of Cercospora sojina Race 1 in Soybean |
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| Abstract: | A F 2 population was generated from a cross between DongNong 9674(the resistant parent) and DongNong 87 104 (the susceptible parent). Following the approach of bulked segregant analysis, eight hundred and twenty RAPD primers were screened to identify the markers linked to the reststance gene Rf1 of Cercospora Sojina race1 in soybean. Three RAPD markers OPK03 840 , OPM17 1700 and OPO10 950 , linked to the resistance gene, were selected and mapped by analyzing F 2 population with the software MAPMARKER II. The positions of the markers relative to the target gene Rf1 are OPK03 840 Rf1 OPM17 1700 OPO10 950 , and the distances are 10 4cM Rf1 13 8cM 26 1cM. |
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| Keywords: | Cercospora sojina Soybean Resistance gene BSA RAPD molecular marker |
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