聚L-谷氨酸光交联水凝胶的制备和性能

以侧链接枝聚乙二醇丙烯酸酯(APEG)的聚L-谷氨酸(PLGA)(PLGA-g-APEG)为前驱体,通过紫外光交联制备水凝胶,研究了APEG的接枝率及PLGA-g-APEG前驱体质量浓度对光交联水凝胶力学性能的影响。结果表明,当APEG接枝率为44.7%,PLGA-g-APEG的质量分数为15%时,水凝胶的力学性能最佳,其储能模量为16238.2 Pa,压缩破坏应力为0.16 MPa。通过多次循环压缩测试,证实压缩曲线几近重合,表明水凝胶具有良好的回弹性能。最后通过MTT法和死/活实验证明PLGA-g-APEG前驱体及其光交联水凝胶均具有良好的生物相容性,该聚L-谷氨酸光交联水凝胶在再生医学领域具有潜在的应用价值。

Fabrication and Characterization of Photo-Crosslinked Poly(L-glutamic acid) Hydrogels

The poly(L-glutamic acid)(PLGA)graft polymer PLGA-g-APEG with a side-linked polyethylene glycol acrylate(APEG)was used as precursor,and the hydrogel was prepared by UV-crosslinking.Then the effects of grafting ratio of APEG and mass concentration of the precursor on the mechanical properties of photocrosslinked hydrogels were studied.When the grafting ratio of APEG reaches 44.7%and the mass fraction of PLGA-g-APEG reaches 15%,the mechanical properties of hydrogel achieve the best.The storage modulus is 16238.2 Pa,and the compressive failure stress is 0.16 MPa.At the same time,the hydrogel was subjected to several cycles of compression test.The results show that the cyclic compression curves are almost completely coincident,the hydrogel has excellent resilience performance.Finally,the results of MTT and the dead/live experiments show that the PLGA-g-APEG precursor and its photocrosslinked hydrogel have good biocompatibility,the hydrogel has potential application in the fields of regenerative medicine.