| 二氢杨梅素锌与牛血清白蛋白的相互作用 |
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| 引用本文： | 严赞开,陈冬丹.二氢杨梅素锌与牛血清白蛋白的相互作用[J].化学与生物工程,2010,27(3):60-62. |
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| 作者姓名： | 严赞开 陈冬丹 |
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| 作者单位： | 韩山师范学院化学系,广东,潮州,521041 |
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| 基金项目： | 广东省科技计划资助项目(2007B031600001) |
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| 摘 要： | 运用荧光光谱法研究了二氢杨梅素锌(DMY-Zn)与牛血清白蛋白(BSA)的相互作用。结果表明,激发波长为288 nm时,BSA的发射峰位于341 nm,DMY-Zn对BSA有较强的荧光猝灭作用。由Stern-Vol mer方程计算出DMY-Zn与BSA体系荧光动态猝灭常数(KSV)可知,DMY-Zn对BSA内源荧光的猝灭机制属于静态猝灭,由Lineweav-er-Burk方程计算出静态猝灭常数为3.180×104L.mol-1;且在BSA分子上荧光敏感部位有1个结合位点,结合常数为9.60×105L.mol-1。
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| 关 键 词： | 二氢杨梅素锌 牛血清白蛋白 荧光光谱 |
Interaction Between Dihydromyricetin-Zinc with Bovine Serum Albumin |
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| YAN Zan-kai,CHEN Dong-dan.Interaction Between Dihydromyricetin-Zinc with Bovine Serum Albumin[J].Chemistry & Bioengineering,2010,27(3):60-62. |
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| Authors: | YAN Zan-kai CHEN Dong-dan |
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| Affiliation: | Department of Chemistry;Hanshan Normal College;Chaozhou 521041;China |
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| Abstract: | Dihydromyricetin-Zn complex(DMY-Zn) was synthesized and characterized by IR spectra.The interaction between DMY-Zn complex with bovine serum albumin(BSA) was investigated by fluorescence spectroscopy.It was shown that DMY-Zn had a powerful ability to quench the BSA fluorescence at excitation and emission wavelength of 288 nm and 341 nm,respectively.The Stern-Volmer quenching constant(KSV) illustrated that the combination reaction of DMY-Zn and BSA was a static quenching process,the Lineweaver-Burk quenching... |
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