营养元素对三角褐指藻生长和脂类积累的影响

研究了不同营养元素(氮、磷、硅、铁)在不同培养浓度条件下,对三角褐指藻生长以及细胞脂类积累的影响作用。结果显示:藻细胞生长随氮含量的增加而增加,但超过一定浓度后作用不明显,最适合三角褐指藻生长的氮含量为 1.76 mmol/L。缺氮和低氮(0.22~0.44 mmol/L)培养能够明显提高藻细胞脂类含量。缺磷培养能有效促进藻株脂类积累,但是藻细胞生长受到明显抑制。缺硅导致藻细胞的生长减缓,但是对藻细胞的油脂积累影响不显著。在缺铁和高铁(0.048 mmol/L)条件下,藻的总脂含量均高于正常的培养条件。实验证明通过两步培养的方法,先在营养充足的培养基中进行藻细胞生长,然后将藻细胞转移到海水中培养,可以明显提高藻细胞的油脂含量。最终本实验确定海水为诱导三角褐指藻脂类积累的最佳诱导培养基。

Effect of Nutrient Elements on Growth and Lipid Accumulation of Phaeodactylum tricornutum

Effects of nutrient elements,such as nitnogen,phosphor,silicon and iron,on growth and lipid accumulation of Phaeodactylum tricornutum were investigated by Nile-red fluorescence method.The results showed that higher nitrogen content would enhance the growth of algal cell and the optimal concentration is 1.76 mmol/L.Nitrogen deficiencies(0.22-0.44 mmol/L) induced the lipid synthesis remarkably.Phosphor deficiencies inhibited the growth of cells,but greatly induced the lipid synthesis.Silicon deficiencies caus...