| 芬顿试剂在土壤DNA提取中的脱腐殖酸效果 |
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| 引用本文： | 宋培勇,梁飞,徐华谷,肖仲久.芬顿试剂在土壤DNA提取中的脱腐殖酸效果[J].腐植酸,2014(5):20-23. |
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| 作者姓名： | 宋培勇 梁飞 徐华谷 肖仲久 |
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| 作者单位： | 遵义师范学院生命科学学院 遵义 563002 |
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| 基金项目： | 贵州省科技厅联合基金项目{黔科合J字LKZS[2012]25号；黔科合J字LKZS[2012]18号}。 |
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| 摘 要： | 为了探索提取土壤DNA之前去除腐殖酸的方法，采用芬顿试剂对土壤样品进行预处理，结果表明芬顿试剂可以有效地去除土壤中的腐殖酸，未经处理和经芬顿试剂处理20～100 min的土壤，均可提取到接近23 kb大小的宏基因组DNA。16S rDNA PCR扩增表明，40～100 min处理土壤提取的DNA可以成功扩增到目的片段，而处理20 min的土壤不一定能扩增到目的片段，未经处理的土壤则不能扩增到目的片段。芬顿试剂可以用于土壤预处理以去除腐殖酸。
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| 关 键 词： | 芬顿试剂 土壤脱腐殖酸 宏基因组 PCR扩增 |
Removal Effect of Humic Acid from Soils before DNA Extraction by Fenton Reagent |
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| Authors: | Song Peiyong Liang Fei Xu Huagu Xiao Zhongjiu |
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| Affiliation: | (School of Life Science, Zunyi Normal College, Zunyi, 563002) |
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| Abstract: | In order to investigate the methods to remove humic acid before DNA extraction from soils, Fenton reagent was employed to carry on pretreatment to soil samples. It showed that metagenome DNA was extracted successfully from all the soils, including the soils untreated and treated for 20 to 100 minutes by Fenton reagent. All the metagenome DNA are close to 23 kb in size. The 16S rDNA fragments were ampliifed successfully based on all the template DNA from soils treated for 40 to 100 minutes, while unsuccessfully for only 20 minutes. Ampliifcation was not obtained for the template DNA from the soils untreated. Therefore, Fenton reagent can be applied to remove humic acid from soils. |
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| Keywords: | Fenton reagent removal of humic acid from soils metagenome PCR ampliifcation |
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