Detection of Listeria monocytogenes in raw whole milk for human consumption in Colombia by real-time PCR |
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| Authors: | Maria Consuelo Vanegas Elizabeth Vásquez Aida Juliana Martinez Adriana M. Rueda |
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| Affiliation: | 1. Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Prince Edward Island C1A 4P, Canada;2. Biogenesis Research Group, Faculty of Agricultural Sciences, University of Antioquia, Calle 70 No. 52-21, C.P. 050010 Medellin, Colombia;1. Department of Microbiology, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, 9 M. Sk?odowska-Curie St., 85-094 Bydgoszcz, Poland;2. Department of Food Hygiene and Consumer Health, Wroc?aw University of Environmental and Life Sciences, 31 C.K. Norwid St., 50-375 Wroc?aw, Poland;3. Department of Microbiology and Food Technology, UTP University of Science and Technology, 6 Bernardyńska St., 85-029 Bydgoszcz, Poland |
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| Abstract: | The aim of our work was to detect Listeria monocytogenes in raw whole milk from five municipalities of Boyacá-Colombia using real-time PCR. A PCR hybridization probe format was used to analyze the presence of L. monocytogenes, using specific primers to amplify a 149 bp fragment from the metalloprotease gene (mpl). In this study on a total number of 81 samples, 21 gave positive results for L. monocytogenes by real-time PCR and 13 samples were positive by conventional method. These results indicate a high presence of this pathogen in this area of the country and that this method is considerably faster than current standard methods. |
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