Authentication of meats from quail (Coturnix coturnix), pheasant (Phasianus colchicus), partridge (Alectoris spp.), and guinea fowl (Numida meleagris) using polymerase chain reaction targeting specific sequences from the mitochondrial 12S rRNA gene |
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| Authors: | María Rojas Isabel González Violeta Fajardo Irene Martín Pablo E Hernández Teresa García Rosario Martín |
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| Affiliation: | 1. Instituto de Investigación en Recursos Cinegéticos, IREC (CSIC, UCLM, JCCM), Ronda de Toledo s/n, 13005 Ciudad Real, Spain;2. Environmental Research Institute, University of the Highlands and Islands, Castle St, Thurso, Caithness, Scotland KW14 7JD, UK;3. IMAR — Instituto do Mar, Department of Life Sciences, University of Coimbra, 3004-517 Coimbra, Portugal;4. Laboratory of Toxicology, Faculty of Veterinary Sciences, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain;1. Tshwane University of Technology, Department of Environmental Health, Faculty of Science, South Africa;2. Stellenbosch University, Department of Animal Sciences, Faculty of AgriSciences, South Africa;3. Tshwane University of Technology, Department of Biotechnology and Food Technology, Faculty of Science, South Africa;4. ARC Biometry Unit, 1134 Park Street, Hatfield, 0083, South Africa;1. Department of Health, Animal Science and Food Safety, Università degli Studi di Milano, Via Celoria, 10, 20133 Milano, Italy;2. Department of Agricultural, Food, and Resource Economics, Michigan State University, USA |
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| Abstract: | Polymerase chain reaction (PCR) based on oligonucleotide primers targeting the mitochondrial 12S rRNA gene has been applied to the specific identification of meats from quail (Coturnix coturnix), pheasant (Phasianus colchicus), partridge (Alectoris spp.), and guinea fowl (Numida meleagris). The use of specific primers pairs for quail, pheasant, partridge and guinea fowl allowed the selective amplification of the desired avian sequences. The specificity of each primer pair was verified by PCR analysis of DNA from meats of various game and domestic bird and mammalian species. The assay can be useful for the accurate identification of meats from game bird species, avoiding mislabelling or fraudulent species substitution in meat products. |
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