Bacterial quality of raw milk investigated by Escherichia coli and isolates analysis for specific virulence-gene markers |
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| Authors: | Abdullah D Altalhi Sabry A Hassan |
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| Affiliation: | 1. Institute National de Recherche Agronomique, Unité de Recherches Fromagères, 15000 Aurillac, France;2. Université de Lyon, Research Group “Bacterial Opportunistic Pathogens and Environment”, UMR5557 Ecologie Microbienne Lyon, Université Lyon 1, CNRS, VetAgro Sup, 69280 Marcy l''Etoile, France;3. Université Paris-Est, ANSES, Laboratoire de Sécurité des Aliments, Plateforme IdentyPath, 14 rue Pierre et Marie Curie, 94701 Maisons-Alfort, France;1. Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt;2. Laboratory of Food Microbiology and Hygiene, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan;1. Department of Food Hygiene and Control, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt;2. Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, MD, USA;3. Department of Environmental Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt;4. Department of Biology and South Texas Center for Emerging Infectious Diseases (STCEID), University of Texas at San Antonio, San Antonio, TX, USA |
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| Abstract: | The bacterial quality and safety of raw milk sources in Taif region (Western Saudi Arabia) were analyzed for the natural contamination of fecal coliform and Escherichia coli by standard most probable number method. The E. coli isolates were identified to species identity using API 20E and screened for markers of STEC (Stx1, Stx2), ETEC (ST, LT), (EaeA) and ExPEC (CNF1, CNF2, SfaS, PapA, CdtB, IutA, FyuA, TraT) using PCR assays. Thirty-three E. coli strains were recovered from raw milk sample sources, which were contaminated by fecal coliform. None of the investigated isolates reacted positively with the PapA, SfaS and CdtB, ST and LT-specific primers. Markers of STEC and EPEC were each detected in three strains. NTEC was detected in four strains. The most frequent virulence markers were TraT (17 strains), IutA (11 strains) and FyuA (8 strains). Results suggested a possibility of potential public health threat of E. coli originating from raw milk sources. |
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